Refrigeration system selection drives roughly 25-35% of total project cost and 60-70% of operating cost over the life of the building. The right system depends on operating temperature, refrigeration tonnage, jurisdiction, refrigerant regulation, operator preference, and noise / setback constraints. There is no universal best answer.
Ammonia (NH3)
Most thermodynamically efficient refrigerant for industrial scale. Cost-competitive first cost. Compliance overhead is real: above 10,000 lb charge, OSHA PSM and EPA RMP apply, adding documentation, engineering, training, mechanical integrity, and audit burden. Setback and ventilation requirements affect site design. Best fit for >200 tons refrigeration on continuous-operation industrial facilities. Read more about ammonia cold storage construction.
CO2 (R-744)
Increasingly common in HFC-regulated jurisdictions and in lower-charge applications. Transcritical and cascade configurations both used. Higher operating pressure means heavier-wall piping. First cost slightly above ammonia at scale; competitive on smaller systems. Good fit for jurisdictions with HFC phase-down (California AB-1346, several Northeast states).
Glycol secondary loop
Ammonia or CO2 primary plant in a sealed machine room, glycol secondary loop into the refrigerated space. Reduces ammonia/CO2 charge in occupied spaces and simplifies compliance. Adds a heat exchanger and pumping energy. Common in cooler-only applications, pharmaceutical, and dock-heavy facilities where charge limits matter.
Ammonia/CO2 cascade
Two-stage cascade with ammonia high side and CO2 low side. Preferred for blast freezing and deep sub-zero applications below -20°F where single-stage ammonia loses efficiency. Higher first cost than single-stage; lower operating cost in deep sub-zero. The standard approach for new blast freezer facilities.
DX (direct expansion)
HFC-based direct expansion. Practical for smaller rooms (under 100 tons refrigeration aggregate), pharmaceutical 2°C-8°C suites where charge limits and serviceability favor packaged equipment, and small-format multi-tenant 3PL. First cost low; operating cost higher than ammonia at scale.